Cryopreserved Cd34 Cells: Measuring Viability Pre-Thaw and Viability over Time Post-Thaw
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چکیده
منابع مشابه
Improving the Post-thaw Viability of Cryopreserved Stallion Spermatozoa
The present study was to improve the success for the cryopreservation of stallion spermatozoa, especially as the costs of using low post-thaw motility or sub-optimal fertility deters owners and breeders from using frozen semen. The aim was to develop a freezing extender/process that supports cell survivability throughout the freezing. Eleven stallions were collected and frozen in INRA 96 with t...
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Cell viability in peripheral blood progenitor cell (PBPC) grafts and its influence on the clinical course following transplantation was evaluated in 81 consecutive transplantations (72 autologous, 9 allogeneic) performed in patients with hematological diseases. Viability of cells in PBPC grafts immediately upon collection was 98.6 +/- 3.5%, after addition of dimethyl sulfoxide (DMSO) 73.3 +/- 2...
متن کاملPost-thaw suspension of red cells cryopreserved with hydroxyethyl starch.
Post-thaw testing of large units of red cells frozen with 14% hydroxyethyl starch (HES) indicates 97-98s of the cells are recovered and about 85% are stable in saline (1). The quantity of supernatant hemoglobin present in such units at thaw is generally near 450-550 mgs. We have been unable to obtain consistent results which reveal a substantial improvement over these values, This quantity of f...
متن کاملSeasonal variations in pre- and post-thaw donor sperm quality.
BACKGROUND The present study was conducted to evaluate seasonal variability in the quality of pre- and post-thaw semen parameters among sperm bank donors. METHODS The first two consecutive ejaculates during the months March (spring, 92 males), June (summer, 97 males), September (autumn, 81 males) and December (winter, 97 males) were analysed. A comparison was made between sperm parameters fro...
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An existing cryopreservation method for liver slices applies 12% dimethylsulfoxide and rapid freezing. We found that cells in rat liver slices cryopreserved in this manner deteriorated rapidly upon culturing. To improve this cryopreservation method, we varied the dimethylsulfoxide concentration (0, 12, 18, and 30%), the cryopreservation medium (Williams medium E, fetal calf serum, and Universit...
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ژورنال
عنوان ژورنال: Biology of Blood and Marrow Transplantation
سال: 2019
ISSN: 1083-8791
DOI: 10.1016/j.bbmt.2018.12.310